| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.060974 |
| Chromosome: | chromosome 16 |
| Location: | 6317402 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g675900 | OAD1 | Arginine/ornithine decarboxylase; (1 of 2) 4.1.1.20 - Diaminopimelate decarboxylase / Meso-2,6-diaminoheptanedioate carboxy-lyase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAATCCAAATTGGCGGCGTGGCAGGCGACAGGCGTGAAGCAAGCCCAGCA |
| Internal bar code: | TTATATGGTAGCCAAGGTTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 735 |
| LEAP-Seq percent confirming: | 25.0 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCCTTCGTACGTATCCGCG |
| Suggested primer 2: | GCATTCTGGAACCGGGTGTA |