| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.060990 |
| Chromosome: | chromosome 16 |
| Location: | 985680 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g648850 | ADS1 | ADP-sulfurylase; (1 of 1) 2.7.7.53 - ATP adenylyltransferase / Diadenosinetetraphosphate alpha-beta-phosphorylase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGTGTTTATGGACAATAATATGTATGCCTTCGAAGGGCGACAGAATAG |
| Internal bar code: | TACAGCTACCCTCTTACCGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1434 |
| LEAP-Seq percent confirming: | 96.1538 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGATGTCCTTTGAGCCCG |
| Suggested primer 2: | CCTGTGTGTCGGTTTTGCTG |