| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.060994 |
| Chromosome: | chromosome 12 |
| Location: | 7290041 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g558450 | SPD1 | Spermidine synthase; (1 of 1) K00797 - spermidine synthase (speE, SRM) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTTTGACTTGGGCAATCCTGGGGGGCTTCGCCCCCCCATCCGGATTGCT |
| Internal bar code: | TTAATCATGCAGTCTCGGGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 6305 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 44 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAACCTCAAGCACACAAGC |
| Suggested primer 2: | AAATTCACACTCGCGCACAC |