| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.060996 |
| Chromosome: | chromosome 5 |
| Location: | 2793620 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g240950 | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGTCAAGCGGGAGGGAAAGGGACACGAGGTGTGTTGGATGGCTCTCCGT |
| Internal bar code: | TAATAGATGATATAGGCTGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4669 |
| LEAP-Seq percent confirming: | 96.6667 |
| LEAP-Seq n confirming: | 145 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 150 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGCACGTACGTATGGCTAG |
| Suggested primer 2: | GTGTTTGAGGGAGCACGAGA |