| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.061001 |
| Chromosome: | chromosome 16 |
| Location: | 3765077 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g684861 | (1 of 781) IPR000104 - Antifreeze protein, type I | CDS | |
| Cre16.g685217 | NUP58 | Nucleoporin 58; (1 of 1) K14307 - nucleoporin p58/p45 (NUPL1, NUP49) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTGTGAACACATCTCGCTACGTATTGGGTTCTGCCTAAGCTGCGAGTG |
| Internal bar code: | AGTTTCAGTTTTAGGGAGCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1426 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCCTGACAGGGGTAGGAT |
| Suggested primer 2: | GTGCAGGTTTGACGCAACAT |