| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.061018 |
| Chromosome: | chromosome 13 |
| Location: | 678613 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g566300 | RRM12,RSMD | (1 of 1) 2.1.1.171 - 16S rRNA (guanine(966)-N(2))-methyltransferase / M(2)G966 methyltransferase; Putative rRNA (guanine-N2-)-methyltransferase | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGAAGGACTGGGTCTGGAGGACCTGGGGCTGGGTCCGGCTGTTGGGCGA |
| Internal bar code: | GTCGTTGGTTTCCGATCATGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4691 |
| LEAP-Seq percent confirming: | 95.0 |
| LEAP-Seq n confirming: | 57 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 60 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAACCTCGTGCCTCAACCAC |
| Suggested primer 2: | GCAATGCCAAACTGTGAGCA |