Insertion junction: CLIP2.061022_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.061022
Chromosome:chromosome 6
Location:6085307
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g290900 (1 of 1) PF06294//PF11971 - CH-like domain in sperm protein (CH_2) // CAMSAP CH domain (CAMSAP_CH) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATTCGACATGTGCTTACGTGGCTGGTGGCTGCCGTGAGAGACGCTTGACA
Internal bar code:TTAACATAGTTTCGACAAGGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:4870
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:29
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:GCAACGAACCAAACAGCCAT
Suggested primer 2:CCGACGTGTTGGGTGTTAGA