Insertion junction: CLIP2.061022_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.061022
Chromosome:chromosome 6
Location:6085307
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g290900 (1 of 1) PF06294//PF11971 - CH-like domain in sperm protein (CH_2) // CAMSAP CH domain (CAMSAP_CH) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTCTTCTAACAGCGTACGAGATGAATCAATGGCTCGTTCGCATCACACT
Internal bar code:TTAACATAGTTTCGACAAGGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:1140
LEAP-Seq percent confirming:92.8571
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:CCGACGTGTTGGGTGTTAGA
Suggested primer 2:GCAACGAACCAAACAGCCAT