| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.061026 |
| Chromosome: | chromosome 2 |
| Location: | 6973907 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g389356 | (1 of 1) K03457 - nucleobase:cation symporter-1, NCS1 family (TC.NCS1) | CDS | |
| Cre09.g389393 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGGGTGAGGGAGAGGCCCACGGGTCTCAGAACAAGCCAACCCCTCAAC |
| Internal bar code: | GGATTACACGCCAGTTGTTTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1007 |
| LEAP-Seq percent confirming: | 97.4359 |
| LEAP-Seq n confirming: | 38 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCCATGCCATGTCAAGCTA |
| Suggested primer 2: | CTAGCAAGGCGGAGGATGAG |