| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.061033 |
| Chromosome: | chromosome 4 |
| Location: | 291258 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g217750 | (1 of 1) PTHR16017//PTHR16017:SF0 - GASTRULATION DEFECTIVE PROTEIN 1-RELATED // WD REPEAT-CONTAINING PROTEIN 70 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAGAGGTAGGAAGGCAGGCAGGAGCAGGGGCAGCATTGTGTGTGCTGT |
| Internal bar code: | AATAACTCCTCGGAGGTCAACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1198 |
| LEAP-Seq percent confirming: | 75.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCAGATTTCCAAGAGCCCT |
| Suggested primer 2: | CAACACGCTCCCATTACCCT |