| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.061035 |
| Chromosome: | chromosome 11 |
| Location: | 4294896 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g482300 | FAP305,MOT17 | Motility 17; (1 of 1) PTHR22706:SF0 - SPERMATOGENESIS-ASSOCIATED PROTEIN 17 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTATCCGACCTGGCGACTGCTTCGCCGGTTGACCAGCTCTTGCCCGAT |
| Internal bar code: | GTGGCGTTCTGGTCATGTAATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3472 |
| LEAP-Seq percent confirming: | 98.6111 |
| LEAP-Seq n confirming: | 71 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 72 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCAGGTTCTGTGGAGGAC |
| Suggested primer 2: | GCAGTACCTCAACAGGACCC |