Insertion junction: CLIP2.061036_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.061036
Chromosome:chromosome 3
Location:186193
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g144104 3'UTR
Cre03.g144124 (1 of 228) IPR029063 - S-adenosyl-L-methionine-dependent methyltransferase 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCTTTTCTCACACATATACAATGTATTATAGTCATGCTGTGAGTTCGCG
Internal bar code:GTTTTTTTCATGCCACGGACAA

Confirmation - LEAP-Seq

LEAP-Seq distance:1483
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:28
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:28

Suggested primers for confirmation by PCR

Suggested primer 1:GGAAGGTGTCACTCCTGTCG
Suggested primer 2:GACGACAACGACACTCCACT