| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.061046 |
| Chromosome: | chromosome 12 |
| Location: | 9461961 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g540750 | (1 of 1) PF05608 - Protein of unknown function (DUF778) (DUF778) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCACACACGGGCTTTGCTCGCGCGCTCCGGCGGACCCAATCCACAGCT |
| Internal bar code: | GCTGACTCCAGCAGGGATTGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 509 |
| LEAP-Seq percent confirming: | 33.3333 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTGACTGTTACGGTCGGG |
| Suggested primer 2: | AAGACCGGCACAACCTACTG |