| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.061056 |
| Chromosome: | chromosome 10 |
| Location: | 69932 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g417950 | (1 of 1) K14824 - ribosome biogenesis protein ERB1 (ERB1, BOP1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGGAGCAGCTTGTGGCGCGAGCTTGACGGCGGGGCTAGCAGCCGGGCG |
| Internal bar code: | ATCGCTAAACGGGGTGAGGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2682 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGGACATGAGGGAGCTC |
| Suggested primer 2: | GACTCAGTGCGTGTGACTCA |