Insertion junction: CLIP2.061064_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.061064
Chromosome:chromosome 9
Location:1573877
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g399000 SDR18 Short-chain dehydrogenase/reductase; (1 of 5) IPR002198//IPR002347//IPR003560//IPR013968 - Short-chain dehydrogenase/reductase SDR // Glucose/ribitol dehydrogenase // 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase // Polyketide synthase, ketoreductase domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTGGCGCCTACCGCCTCAATTCCTCTGGCACCAGGTGATCAACTACAAC
Internal bar code:CGGGGGATTTAATGATCCAACA

Confirmation - LEAP-Seq

LEAP-Seq distance:1456
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:28
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:28

Suggested primers for confirmation by PCR

Suggested primer 1:GGACATCAGGTGGAGCTCAC
Suggested primer 2:AAGGGGACTAGCTCCGTGAT