| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.061079 |
| Chromosome: | chromosome 2 |
| Location: | 4862105 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g109550 | HSD1,AHD3 | Homoserine dehydrogenase; (1 of 2) K12524 - bifunctional aspartokinase / homoserine dehydrogenase 1 (thrA) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGGAATGATTCCACCATAATACTGGCGGCCCGAATGCCCGATTCGATC |
| Internal bar code: | TCAACTTATCGGTAATCACGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2733 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 103 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 103 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGCCGCGATTTAGTCAAG |
| Suggested primer 2: | GACCGGGGAACACTTGTCAT |