Insertion junction: CLIP2.061085_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.061085
Chromosome:chromosome 14
Location:2747359
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre14.g626200 P4H7,PFH7,PHX18 Prolyl 4-hydroxylase 7; (1 of 14) K00472 - prolyl 4-hydroxylase (E1.14.11.2) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGTGGTGAGCGCCGCTCCTGCCCGTTGCGGCAAGTGCCGCAGGTCCGGG
Internal bar code:GAAAATTCGGTAGATGTTTTCC

Confirmation - LEAP-Seq

LEAP-Seq distance:856
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:CGTGTTGTGCTGTTGAGTGG
Suggested primer 2:GGAGGCCTGAACGTGAGAAA