| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.061093 |
| Chromosome: | chromosome 10 |
| Location: | 4255405 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g449700 | (1 of 7) PF00582 - Universal stress protein family (Usp) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAAGAGCGAGAAGTATCGTTTAGTTGTGTATATGCGGGAAGCTCGATGC |
| Internal bar code: | ATTAGTTCCCCACACTGTGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2742 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 56 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 56 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACTTCAGGTTGCGTTGGG |
| Suggested primer 2: | CGATCATAGCCTGCAGAGCA |