| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.061099 |
| Chromosome: | chromosome 9 |
| Location: | 6746991 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g417150 | CAT1 | Mono-functional catalase; (1 of 1) 1.11.1.6 - Catalase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGTACTGTACTAGAAATCCTAGTACGCTGTATTAATACGCGTATTATGG |
| Internal bar code: | ATAAGATATAAAAATGACCTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2842 |
| LEAP-Seq percent confirming: | 92.5 |
| LEAP-Seq n confirming: | 74 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 80 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACTCGGTCTCAGCACAACA |
| Suggested primer 2: | CAAAGTCTCGCCCCAGACAT |