Insertion junction: CLIP2.061108_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.061108
Chromosome:chromosome 16
Location:2399784
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g659600 (1 of 1) K14834 - nucleolar complex protein 3 (NOC3) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTCTTAGCGCATCGTCAATGACTGCTGTTCTGTCCTCTCCTTCTGGGCC
Internal bar code:GTAAGTGCGATTTTCGGAGTAA

Confirmation - LEAP-Seq

LEAP-Seq distance:1791
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:21
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:GACGCCCGGTTTTATTTCGG
Suggested primer 2:CACATTGCATCGCATACGCA