| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.061131 |
| Chromosome: | chromosome 17 |
| Location: | 3329345 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g722650 | 3'UTR | ||
| Cre17.g802066 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGCTCAATCAAATGAGCAAGGTCTTACCACATTTGTAAGGCTGTCCCG |
| Internal bar code: | GTTGAGGTTAGGAGTGGTATAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 526 |
| LEAP-Seq percent confirming: | 24.3902 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGGAGGAGTTTGTGTAGGC |
| Suggested primer 2: | TGCCTGTGCGTAGTAAAGGG |