Insertion junction: CLIP2.061135_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.061135
Chromosome:chromosome 6
Location:6072402
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g290800 RMG1 RNA cap guanine-N2 methyltransferase; (1 of 1) 2.3.1.221 - Noranthrone synthase / Polyketide synthase A 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGGTGTAAGGTCCCACATACATCCTCTAATCCTCTTTCAGCCAACTGCA
Internal bar code:CTGTTTTCATGAAAAAGCATTA

Confirmation - LEAP-Seq

LEAP-Seq distance:2298
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:41
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:41

Suggested primers for confirmation by PCR

Suggested primer 1:GCTGTGTAAGAGTCCGCACT
Suggested primer 2:CAATACGCGTGCGCACAATA