| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.061137 |
| Chromosome: | chromosome 6 |
| Location: | 4913190 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g280800 | (1 of 1) K11291 - nuclear autoantigenic sperm protein (NASP) | 3'UTR | |
| Cre06.g280850 | PBG1 | 20S proteasome beta subunit, type 4; (1 of 1) K02736 - 20S proteasome subunit beta 7 (PSMB4) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCTCCAGGCGGCCCTGCCACTGAGGTGTGCACCACCAAGCCGGAACCG |
| Internal bar code: | GTTTGTCGTGACGGTCTGGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1740 |
| LEAP-Seq percent confirming: | 96.875 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACAAGTTCCAGGTGGCCAA |
| Suggested primer 2: | GTTTCTGATGGCCTGGGACA |