| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.061138 |
| Chromosome: | chromosome 1 |
| Location: | 6073470 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g042900 | RRM1 | Putative rRNA (adenine-N6-)-methyltransferase; (1 of 1) 2.1.1.183 - 18S rRNA (adenine(1779)-N(6)/adenine(1780)-N(6))-dimethyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTATGCGCGCAGCGCGCCACGGATGTGGGTCGGGGGCAGAATGGTGCGG |
| Internal bar code: | AAAGAAAGGCCGTGGACGGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 944 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 34 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTAGCCATACGTCGGACTG |
| Suggested primer 2: | GGTAAGGGAGCCAAGGTGTC |