Insertion junction: CLIP2.061145_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.061145
Chromosome:chromosome 11
Location:1941199
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g467780 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCAAAGTCTGATCAATACGTTGATCCCAGCCCAGATTCGGGCGGCAGCG
Internal bar code:GAAGGTTATCAACTCGTACGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:357
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:23
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:GTGTGTGTGTGTGCGACATC
Suggested primer 2:CGTAGGAGACCGCTGTGATC