Insertion junction: CLIP2.061152_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.061152
Chromosome:chromosome 17
Location:4072398
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g728750 (1 of 40) 3.4.24.38 - Gametolysin / Lysin 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGATGAATAGAAGGTTCTCCACAGACGGGCCACACCCTACTCCAGCAAG
Internal bar code:ATTTACATTTGTTGAAGATCCA

Confirmation - LEAP-Seq

LEAP-Seq distance:2716
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:GCAGAGACAGCAGGCAAGTA
Suggested primer 2:AACAACATCAACCGGCAAGC