Insertion junction: CLIP2.061157_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.061157
Chromosome:chromosome 8
Location:210798
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g358554 (1 of 14) 3.1.3.16//3.1.3.48 - Protein-serine/threonine phosphatase / Serine/threonine specific protein phosphatase // Protein-tyrosine-phosphatase / PTPase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGAAGTTGAGTTTGGTTTAATGGTTCCTCGGTTGGTGTCGGACATGTCGG
Internal bar code:AGTATCAAACGATATATTCTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:867
LEAP-Seq percent confirming:81.5789
LEAP-Seq n confirming:31
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:38

Suggested primers for confirmation by PCR

Suggested primer 1:CCATCACTACCTGCAGCCAA
Suggested primer 2:CCACCCAACCCCAACACATA