| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.061167 |
| Chromosome: | chromosome 7 |
| Location: | 378077 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g314700 | (1 of 1) PF14675 - FANCI solenoid 1 (FANCI_S1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGATCACCTCTCCATAGCTCAGCTGGAAGAGCGAAGGACTGTAATTGTT |
| Internal bar code: | GTCTCAATTTTTTAAGCACGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3232 |
| LEAP-Seq percent confirming: | 92.4242 |
| LEAP-Seq n confirming: | 61 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 66 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTCTGCCCTGGACTTTGG |
| Suggested primer 2: | ATGACGAGTGTTGCGGAACT |