| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.061172 |
| Chromosome: | chromosome 16 |
| Location: | 6625967 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g673852 | (1 of 2) K01595 - phosphoenolpyruvate carboxylase (ppc) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATTGAAAGATCGCTAAAGAAAGAAAAATTCAGCGGTTATCAGCCCGCGC |
| Internal bar code: | GAATCTAATTACTCACGTCGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1621 |
| LEAP-Seq percent confirming: | 78.125 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGATTCAAGTGACATCCGC |
| Suggested primer 2: | AAGCTGAGCAAACAAGCGTG |