| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.061191 |
| Chromosome: | chromosome 12 |
| Location: | 514835 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g494252 | (1 of 1) PF14822 - Vasohibin (Vasohibin) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTATTAGCCCGCGCGGTCTCTTTGTCAGCCCCAGCTAAGAGATTCGCT |
| Internal bar code: | TAAGATGTGTAATGGGCTTATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1204 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCCCCCGCGTTAGTAAATT |
| Suggested primer 2: | GACTTACACGCGTCACAGGA |