Insertion junction: CLIP2.061194_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.061194
Chromosome:chromosome 17
Location:3290016
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g722100 SSA3 cilia-sensing, structure and/or assembly; (1 of 1) PTHR12771:SF2 - ELMO DOMAIN-CONTAINING PROTEIN 3 3'UTR
Cre17.g722150 PKS3 (1 of 1) 2.3.1.119//2.3.1.199 - Transferred entry: 1.1.1.330, 1.3.1.93, 2.3.1.199 and 4.2.1.134 // Very-long-chain 3-oxoacyl-CoA synthase / Very-long-chain beta-ketoacyl-CoA synthase; Type III polyketide synthase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCGAGGTGACGCCGACTACCGTCTACACTGAATGTAATTCAAGCACTCA
Internal bar code:TGTCCAGGAGTGGGGGGTTGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:1354
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:76
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:76

Suggested primers for confirmation by PCR

Suggested primer 1:GTCTTGGGGAAGGGTTCGAG
Suggested primer 2:ATACTGGAAGGTTGTGCGGG