| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.061195 |
| Chromosome: | chromosome 4 |
| Location: | 2259270 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g220424 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCTAAGCGAAACCATTCAAAGCGTATGCTGAGGACCTCGGTTTTTAAC |
| Internal bar code: | GCCCACTAATAGGTAAGATGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 452 |
| LEAP-Seq percent confirming: | 76.1905 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGACACCTTCGACTTCCC |
| Suggested primer 2: | CGTGCGCTTGTGATAAGTCG |