| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.061219 |
| Chromosome: | chromosome 17 |
| Location: | 5447074 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g738850 | (1 of 1) IPR000104//IPR003169 - Antifreeze protein, type I // GYF domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAATTTTGGCAGTCGCAGCTGCCCACCCGTGTGTGCTAATGCGCGCGCG |
| Internal bar code: | CGCTGCAGTCATAGATTTTAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 655 |
| LEAP-Seq percent confirming: | 60.4651 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCCGCAGCTGGCTCAAATA |
| Suggested primer 2: | CAAGGAGTTGCACAAGCCAC |