| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.061231 |
| Chromosome: | chromosome 3 |
| Location: | 6715329 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g195900 | (1 of 7) PTHR11584//PTHR11584:SF381 - SERINE/THREONINE PROTEIN KINASE // PROTEIN PQN-80, ISOFORM B | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGGCCGCCAAAGTTGGGGGGTCGGTCCTCGTCCCTGGGGGTCCGTCGG |
| Internal bar code: | TGGTGTACGTGCGGTCAGCCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2254 |
| LEAP-Seq percent confirming: | 59.0909 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACAAAGTGCCAGACCCCA |
| Suggested primer 2: | GTGGGTCAACGCAAAGGTTC |