| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.061237 |
| Chromosome: | chromosome 12 |
| Location: | 3517678 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g496750 | NUO8 | (1 of 1) K03941 - NADH dehydrogenase (ubiquinone) Fe-S protein 8 (NDUFS8); NADH:ubiquinone oxidoreductase subunit 8 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCCTGCCGCTGCCGCGCTCACCGCCTCGCACAGCTTGCAGGCGATGCAG |
| Internal bar code: | TCTGGTTTATTGTAGTGCGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 290 |
| LEAP-Seq percent confirming: | 33.3333 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGATGCTGCTACTGACGGAT |
| Suggested primer 2: | TTGCACCAGAATCGTGTCGA |