| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.061244 |
| Chromosome: | chromosome 12 |
| Location: | 5145336 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g526650 | (1 of 8) IPR000719//IPR002290//IPR011009//IPR020635//IPR020636 - Protein kinase domain // Serine/threonine/dual specificity protein kinase, catalytic domain // Protein kinase-like domain // Tyrosine-protein kinase, catalytic domain // Calcium/calmodulin-dependent/calcium-dependent protein kinase | 3'UTR | |
| Cre12.g526700 | RBL9 | Rhomboid-like protease; (1 of 11) 3.4.21.105 - Rhomboid protease | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGAAGCCTGCTGCCAGATCCATGCCAAACGACACGCTCTCCAAATACCC |
| Internal bar code: | AGAACTCTTTTCTTGCTAGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3400 |
| LEAP-Seq percent confirming: | 77.0492 |
| LEAP-Seq n confirming: | 47 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 61 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTGACGGGTTGCAAGTAG |
| Suggested primer 2: | CAGGCACGCACAATTGAACA |