| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.061252 |
| Chromosome: | chromosome 10 |
| Location: | 775162 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g422800 | HER1 | DnaJ-domain protein; (1 of 1) PF00226//PF00570 - DnaJ domain (DnaJ) // HRDC domain (HRDC) | 5'UTR |
| Cre10.g422850 | (1 of 5) PTHR11614:SF87 - MONOGLYCERIDE LIPASE | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTTAGCAGCCCTGAGGTCGCGGTGACGGTCGCGGTCGCCGTCCTATTAA |
| Internal bar code: | AGGGCTTGGGATGTAAAGCAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2675 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 65 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 65 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGCCCTCTTAGCTTCACAA |
| Suggested primer 2: | GCGCCAGCTTTCTCATATGC |