Insertion junction: CLIP2.061258_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.061258
Chromosome:chromosome 6
Location:3090069
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g275200 3'UTR
Cre06.g275250 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCGCGGTTGACCCGCGGCTTGTATAGGACTGTGGGCTGGGCGCGCGCG
Internal bar code:GACACTGAGGTGATAGATGTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:1344
LEAP-Seq percent confirming:93.0233
LEAP-Seq n confirming:40
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:43

Suggested primers for confirmation by PCR

Suggested primer 1:CCACAGTTGCAGCAGGAAAC
Suggested primer 2:CAGTGTGGAGGTGTCAGGTC