| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.061285 |
| Chromosome: | chromosome 10 |
| Location: | 4479220 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g450900 | (1 of 6) PF09353 - Domain of unknown function (DUF1995) (DUF1995) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCGCTCCTGATTTGTATTATTTACAGACGGCATTGCGCCCATGTGGGT |
| Internal bar code: | CGGCGAGGAAAATCGATTTGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4467 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 80 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 80 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGAAACCCACAACAGCTGT |
| Suggested primer 2: | CCATCCACCCCAACCCTTAC |