Insertion junction: CLIP2.061292_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.061292
Chromosome:chromosome 9
Location:2540134
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g392750 (1 of 1) PTHR10972:SF45 - OXYSTEROL-BINDING PROTEIN 9 3'UTR
Cre09.g392800 POB24 Proteome of basal body 24 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGTACATCGCCGCAACGCACTCCAACGGCTCACTTCCTCGCGACATTTG
Internal bar code:ACGGTCTCTGCTGGGCAGGTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:1163
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:27
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:27

Suggested primers for confirmation by PCR

Suggested primer 1:TAGCGGTGTGTGTGACTGAC
Suggested primer 2:TTGCCAGCCAGTCTTCGAAT