| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.061313 |
| Chromosome: | chromosome 13 |
| Location: | 1450534 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g572150 | NAT29 | N-acetyltransferase; (1 of 1) PTHR14744:SF15 - N-ALPHA-ACETYLTRANSFERASE 60 | 3'UTR |
| Cre13.g572200 | (1 of 1) K03834 - tyrosine-specific transport protein (tyrP) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTAGTTTGAGGACTGTTGATGGGCAGGACAGAACACTGAGCAGGGCGGG |
| Internal bar code: | TTTGGGGGGTATCGGCCAAAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1735 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGATGAGGTAGACGCTGGC |
| Suggested primer 2: | CTACCGGGATAGGCAACGTC |