| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.061322 |
| Chromosome: | chromosome 7 |
| Location: | 5638234 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g352450 | SDR10 | Short-chain dehydrogenase/reductase; (1 of 3) 1.1.1.30 - 3-hydroxybutyrate dehydrogenase / D-beta-hydroxybutyrate dehydrogenase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCACCATGCTACGGGTTGGCGCCAACCATTCGGGCCAGGGCTGCGGAG |
| Internal bar code: | GTTTTGAACGACACGCCGCCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1401 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 40 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCACCTGCATTGTTCACAA |
| Suggested primer 2: | CTCACGTGTCTGTGACGTCA |