| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.061327 |
| Chromosome: | chromosome 2 |
| Location: | 2533716 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g092284 | (1 of 1) 1.13.11.19 - Cysteamine dioxygenase / Persulfurase | 5'UTR | |
| Cre02.g092350 | CYP51G1,CYP6 | (1 of 1) 1.14.13.70 - Sterol 14-alpha-demethylase / Obtusufoliol 14-demethylase; Cytochrome P450, CYP51 superfamily | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTATGATATTGGATGTCACAACGCGCCGCGATGAACAGAGTAACTCGG |
| Internal bar code: | CCTGTACGTTCTCGCTATTGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4304 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCGTCAACTGAGGTCAAGC |
| Suggested primer 2: | GCAGATAGGACGAGGCTGTC |