Insertion junction: CLIP2.061328_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.061328
Chromosome:chromosome 14
Location:1955068
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g620702 PHC24 (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin; Pherophorin-chlamydomonas homolog 24 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGGCGAAGCTGTGCGCCGTGGCTGTAGCGGCTGCTGCCTCTGCTGCCAA
Internal bar code:GACTTCTAGAGCACGTGTGGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:1296
LEAP-Seq percent confirming:94.4444
LEAP-Seq n confirming:34
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:36

Suggested primers for confirmation by PCR

Suggested primer 1:CGCAACAACGTACTTGCCAA
Suggested primer 2:CAGAGCTTTCGCCGTTATGC