Insertion junction: CLIP2.061337_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.061337
Chromosome:chromosome 3
Location:2164412
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g157000 ELG1 Exostosin-like glycosyltransferase 1; (1 of 1) PF12661 - Human growth factor-like EGF (hEGF) 3'UTR
Cre03.g157050 (1 of 1) IPR020966 - Aluminum-activated malate transporter 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTCTGCCGTGCCAGTGTCTCCGTTCAGGTGTTATATTGTGCTTGCAGCC
Internal bar code:AGTGTTGGACTTTATGGGCTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:2335
LEAP-Seq percent confirming:95.2381
LEAP-Seq n confirming:20
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:CTTGGTCTGTCACGCTGTCT
Suggested primer 2:TACGGACGTACAGGGGCTTA