| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.061347 |
| Chromosome: | chromosome 8 |
| Location: | 1809212 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g366874 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCAACAGGGTTACCGAAGATGCTTAGATGAAGGTGCACACGGTGGATG |
| Internal bar code: | ACCGGGGGCTGTCCACGCAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2167 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAGGTACCTATCGGTGCAC |
| Suggested primer 2: | TATCCAACCAGCGTGAACCC |