| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.061348 |
| Chromosome: | chromosome 12 |
| Location: | 8792221 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g547000 | (1 of 1) IPR003442//IPR027417 - tRNA threonylcarbamoyl adenosine modification protein TsaE // P-loop containing nucleoside triphosphate hydrolase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCGCTGGAGCGAATGGCTGCACGGTGTGCCCACCCGGTAGCGGCAGTGT |
| Internal bar code: | CTGGGCCTGTACTGGATCCATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3571 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 64 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 64 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCGGCAAGAAGAAGTCCTC |
| Suggested primer 2: | AGGATGACTTTCTGCCGGTG |