| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.061350 |
| Chromosome: | chromosome 7 |
| Location: | 6065457 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g355250 | WEE1 | CDK inhibitory kinase; (1 of 1) K06632 - wee1-like protein kinase (WEE1) | 5'UTR |
| Cre07.g355300 | BES5 | (1 of 10) PF01062 - Bestrophin, RFP-TM, chloride channel (Bestrophin); putative chloride channel | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGACATCAGTTCATGGATCAGGCAGATATCCATAGAAGGTATCACTCAA |
| Internal bar code: | TGTTGGGTGGACTGCTGTGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2907 |
| LEAP-Seq percent confirming: | 98.8636 |
| LEAP-Seq n confirming: | 87 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 88 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTCAGGTTCTCAGTGCAC |
| Suggested primer 2: | CGAGATGTCGCCGAGATTCA |