Insertion junction: CLIP2.061354_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.061354
Chromosome:chromosome 15
Location:398881
Confidence (%):40
Locus systematic id Locus common name Defline Feature
Cre15.g640901 (1 of 1) IPR000104//IPR029033 - Antifreeze protein, type I // Histidine phosphatase superfamily intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACACACACACACACACACACACACACACACACACACACACACACACACAC
Internal bar code:TTTCGGATAGCGTCTACGAGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:408
LEAP-Seq percent confirming:15.625
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:27
LEAP-Seq n unique pos:32

Suggested primers for confirmation by PCR

Suggested primer 1:TGCCATGAACTCGATGCTGT
Suggested primer 2:AAACCAACGAAAACGAGCGG