| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.061356 |
| Chromosome: | chromosome 16 |
| Location: | 1687072 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g801893 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATAACCCCGGACCCTTGCCGGCCACGGCCGCTTGTGTTTAAAGGGAAGAG |
| Internal bar code: | GGGATCTTTGGAATGATGTCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 360 |
| LEAP-Seq percent confirming: | 11.1111 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 72 |
| LEAP-Seq n unique pos: | 81 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGGCGGTCTATCATCAGG |
| Suggested primer 2: | ACGGTATGTCGCACCAACAT |